Narishige Pc10 Manual New 2021 Page
The final shape of the pipette is determined by three main variables:
: Stabilized internal power supply regulating a platinum/kanthal heating coil filament.
The PC-10 is a vertical, two-stage puller designed to create sharp micropipettes with consistent tip diameters and tapers. Its vertical design utilizes gravity, reducing the influence of friction compared to horizontal pullers, which helps in achieving highly reproducible results.
The Narishige PC-10 is a dual-stage vertical glass micropipette puller widely used in electrophysiology, microinjection, and cellular manipulation. This comprehensive guide serves as an operational and technical manual for the PC-10 model, detailing installation, parameter adjustment, and routine troubleshooting. 1. Product Overview and Technical Specifications
For patch-clamp pipettes, standard weight configurations usually involve utilizing the light or medium weight set. narishige pc10 manual new
Consistency can vary with lab humidity; keep a log of settings that work for your specific environment. ResearchGate
The process stops after an initial "softening" pull and then performs a second, more intense pull. This is essential for producing firm microelectrodes with the sharp tips (0.1µm to several µm) required for patch clamp experiments. 3. Setting Your Parameters
The updated Narishige PC-10 manual includes the following new features:
: The secondary heater ( NO. 2 HEATER ) fires automatically to draw out the final tip shape. The final shape of the pipette is determined
Thread the glass capillary vertically through the center of the platinum heating coil.
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Proper physical placement and environmental stability are critical to achieving reproducible pipette shapes. Environmental Requirements
1.0 mm to 2.0 mm outer diameter (OD) glass capillaries. The Narishige PC-10 is a dual-stage vertical glass
Gradually increase the Heater 1 and Heater 2 dials in small increments. Heater settings are too high.
[Single-Stage Pull] ─── Heat Activated ─── Continuous Gravity Drop ───► Long, Flexible Taper (Microinjection) [Two-Stage Pull] ─── First Heat ────► Controlled Stop ───► Second Heat ───► Firm, Short Shank (No.1 Level) (Adjustable Slider) (No.2 Level) (Patch Clamp) One-Stage (Single) Pull Mode
The unit includes two light (approx. 25g) and two heavy (approx. 100g) weights. More Weight: Results in a steeper taper and sharper edge. Less Weight: Results in a thinner, more parallel tip. NARISHIGE WEB NEWS 3. Step-by-Step Operation Mount the Capillary: Place a glass capillary (e.g., ) into the heater coil and secure it with the clamps. Select Mode: for simple needles or for firm microelectrodes used in patch clamping. Adjust Heating: No. 1 Heater knob to your desired value (start around 60 for reference). Set Tension:
The platinum heater is a consumable part. Over time, it will oxidize or break.